Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subcortical Foundations of Binocular Vision: Circuits and Computation from Retina to Cortex and Back.

Annual review of vision science·2026
Same author

Nonlinear KCCA in fMRI activation analysis: Self-supervised optimization and robust back-reconstruction.

Imaging neuroscience (Cambridge, Mass.)·2026
Same author

Comprehensive Lineage Tracing Maps the Landscape of Cell Fate Decisions in Mouse Embryogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Advancing biomarker development for chronic traumatic encephalopathy: Summary and recommendations from the 2025 Leon Thal Summit.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Age-varying DNA methylation patterns associated with blood pressure in mid-to-late adulthood.

Clinical epigenetics·2026
Same author

Spatial organization and detection of social odors in mouse primary olfactory system.

Cell·2026

Related Experiment Video

Updated: Apr 1, 2026

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

6.4K

Mapping Synaptic Input Fields of Neurons with Super-Resolution Imaging.

Yaron M Sigal1, Colenso M Speer1, Hazen P Babcock2

  • 1Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Cell
|October 6, 2015
PubMed
Summary

Researchers mapped inhibitory synaptic inputs in mouse retinal neurons using a novel super-resolution platform. This revealed specific GABAergic inputs crucial for visual motion detection in On-Off direction-selective ganglion cells (DSGCs).

More Related Videos

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

16.1K
Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.2K

Related Experiment Videos

Last Updated: Apr 1, 2026

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
14:02

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

6.4K
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

16.1K
Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.2K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Visual System Research

Background:

  • Neurons integrate thousands of synaptic inputs, making synaptic mapping crucial for understanding neural computation.
  • Characterizing synaptic input fields, including identity, strength, and location, is essential for deciphering neuronal function.
  • On-Off direction-selective ganglion cells (DSGCs) in the retina are key for processing visual motion direction.

Purpose of the Study:

  • To develop a volumetric super-resolution reconstruction platform for large-volume neural imaging and synapse segmentation.
  • To map the inhibitory synaptic input fields of On-Off DSGCs in the mouse retina.
  • To understand the molecular identity and receptor subtype specificity of synapses contributing to direction selectivity.

Main Methods:

  • Development of a novel volumetric super-resolution reconstruction platform.
  • Automated segmentation of neurons and synapses with molecular identity.
  • Application of the platform to image and analyze synaptic inputs onto On-Off DSGCs.

Main Results:

  • The platform enabled detailed reconstruction of large neural volumes with molecular information.
  • Mapping revealed that On-Off DSGCs receive specific GABAergic inputs.
  • These GABAergic inputs, with specific receptor subtypes, are critical for generating direction-selective responses, without significant glycinergic input for monosynaptic crossover inhibition.

Conclusions:

  • The developed super-resolution platform offers unique capabilities for interrogating complex neural circuitry at high resolution.
  • The study elucidates the specific inhibitory synaptic mechanisms underlying visual motion detection in DSGCs.
  • Findings highlight the importance of GABAergic signaling and receptor specificity in neural computation of visual direction.