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Updated: Apr 1, 2026
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
Published on: September 13, 2022
In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
Ryan M Williams1, Letha J Sooter2
1Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry, 1275 York Avenue, New York, NY 10065, USA ; Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Abstract:
Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucleic or amino acids. This review outlines the construction of peptide and antibody fragment libraries as well as their different host types. Common methods of selection are also reviewed. Additionally, examples of cancer cell MREs are discussed, as well as their potential applications.
