Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

804
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
804
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.8K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.8K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

853
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
853
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.7K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

908
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
908
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.6K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of 1,3-Disubstituted 3-Azabicyclo[3.2.0]heptane Libraries for Fragment-Based Drug Discovery.

Organic letters·2026
Same author

Mechanochemical Methods for Amide Bond Formation.

Chemical reviews·2026
Same author

Synchrotron X-rays elucidate the intracellular storage of the iodide and bromide antioxidant system in the kelp Laminaria digitata.

Journal of the Royal Society, Interface·2026
Same author

Moving NMR infrastructures to remote access capabilities.

Progress in nuclear magnetic resonance spectroscopy·2026
Same author

Detecting glycosyl-oxonium and glycosyl-nitrilium ions using exchange NMR to investigate solvent effects in glycosylation reactions.

Nature communications·2026
Same author

In situ NMR-guided design of alkaline electrochemical ammonia synthesis.

Science advances·2025

Related Experiment Video

Updated: Apr 1, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.2K

2D NMR Trace Analysis by Continuous Hyperpolarization at High Magnetic Field.

Nan Eshuis1, Ruud L E G Aspers1, Bram J A van Weerdenburg1

  • 1Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, Nijmegen, 6525 AJ (Netherlands).

Angewandte Chemie (International Ed. in English)
|October 7, 2015
PubMed
Summary

This study introduces a new hyperpolarization method for nuclear magnetic resonance (NMR) that significantly boosts sensitivity. The technique enables precise detection and quantification of trace analytes in complex mixtures at nanomolar concentrations.

Keywords:
hyperpolarizationmultidimensional NMR spectroscopypara-hydrogensignal amplificationtrace analysis

More Related Videos

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K
Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

20.3K

Related Experiment Videos

Last Updated: Apr 1, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.2K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K
Hyperpolarized Xenon for NMR and MRI Applications
16:20

Hyperpolarized Xenon for NMR and MRI Applications

Published on: September 6, 2012

20.3K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Physical Chemistry

Background:

  • Nuclear magnetic resonance (NMR) is a powerful analytical technique due to its sensitivity, quantitative nature, and simple sample preparation.
  • However, its application in detecting trace analytes in complex mixtures is hindered by low sensitivity and signal overlap.

Purpose of the Study:

  • To develop an advanced NMR approach for enhanced detection and quantification of trace analytes.
  • To overcome the limitations of conventional NMR sensitivity and signal resolution in complex samples.

Main Methods:

  • Implementation of continuous hyperpolarization at high magnetic field using Signal Amplification By Reversible Exchange (SABRE).
  • Integration of the SABRE technique into multidimensional NMR experiments, specifically a 2D correlation experiment.

Main Results:

  • Demonstrated detection and quantification of analytes at nanomolar concentrations.
  • Successfully applied the enhanced NMR method to complex solutions, overcoming traditional sensitivity limitations.

Conclusions:

  • The developed continuous hyperpolarization approach significantly improves NMR sensitivity for trace analyte analysis.
  • This method offers a viable solution for precise quantification in complex mixtures, expanding the utility of NMR spectroscopy.