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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Easy and Accurate Mechano-profiling on Micropost Arrays

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Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic

Soo Hyeon Kim¹, Maria Antfolk², Marina Kobayashi¹

¹Institute of Industrial Science, The University of Tokyo, Japan. tfujii@iis.u-tokyo.ac.jp and CREST, Japan Science and Technology Agency, Japan.

|October 7, 2015

Summary

This study introduces a novel microfluidic system for high-throughput single-cell arraying of rare cells. The integrated acoustofluidic and electroactive microwell system significantly enhances throughput and cell recovery for rare cell analysis.

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Area of Science:

Microfluidics
Cell Biology
Biotechnology

Background:

High-throughput single-cell analysis is crucial for rare cell studies.
Volumetric throughput limitations hinder the analysis of rare cells in large sample volumes.

Purpose of the Study:

To develop a novel microfluidic system for high-throughput single-cell arraying of rare cells.
To overcome the throughput bottleneck in rare cell isolation and analysis.

Main Methods:

Integration of an acoustofluidic chip with an electroactive microwell array.
Utilizing acoustofluidics for cell velocity reduction and dielectrophoresis for cell trapping.
Serial integration of acoustophoretic pre-concentration and dielectrophoretic cell trapping.

Main Results:

Achieved a 10-fold improvement in sample throughput compared to using the electroactive microwell array alone.
Maintained a high cell recovery rate exceeding 90%.
Demonstrated drastically improved performance for rare cell analysis.

Conclusions:

The integrated system offers a simple and effective solution for high-throughput single-cell arraying.
This approach significantly enhances the performance of electroactive microwell arrays for rare cell analysis.
Potential for development into a highly integrated and automated platform for rare cell analysis, including sorting and downstream applications.