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Targeted Mutagenesis in Zebrafish by TALENs
Peng Huang1, An Xiao1, Xiangjun Tong1
1Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing, 100871, P. R. China.
Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2015
Summary
This chapter details TALENs (TALE nucleases) for zebrafish genome editing. Protocols cover gene disruption, large deletions, and precise modifications via homologous recombination (HR) for disease modeling and regeneration studies.
Area of Science:
- * Molecular Biology and Genetics
- * Zebrafish as a Model Organism
Background:
- * Zebrafish are crucial for studying vertebrate development, organ regeneration, and human diseases.
- * TALE nucleases (TALENs) are powerful tools for genome editing in zebrafish research.
Purpose of the Study:
- * To provide detailed protocols for TALEN-mediated genome manipulation in zebrafish.
- * To enable targeted gene disruption, large genomic region deletion, and precise gene editing.
Main Methods:
- * Utilizing TALE nucleases (TALENs) for targeted genome modifications.
- * Implementing indel mutations for gene disruption.
- * Employing dual TALEN pairs for large genomic region deletion.
- * Leveraging homologous recombination (HR) for precise genome editing.
Main Results:
- * Established detailed protocols for various TALEN-based genome editing strategies in zebrafish.
- * Demonstrated successful gene disruption, deletion, and precise modification.
- * Facilitated the generation of zebrafish disease models and studies on regeneration.
Conclusions:
- * TALENs offer versatile and effective methods for zebrafish genome engineering.
- * These protocols advance the use of zebrafish in genetic studies, disease modeling, and regenerative biology.
