Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chromatin spatial analysis by METALoci unveils sex-determining 3D regulatory hubs.

Nature structural & molecular biology·2026

Same author

TREM2 expression level is critical for microglial state, metabolic capacity and efficacy of TREM2 agonism.

Nature communications·2026

Same author

Personalized CRISPR knock-in cytokine gene therapy to remodel the tumor microenvironment and enhance CAR T cell therapy in solid tumors.

Nature communications·2025

Same author

BACE1 regulates sleep-wake cycle through both enzymatic and non-enzymatic actions.

EMBO reports·2025

Same author

Prime editing corrects the dilated cardiomyopathy causing RBM20-P633L-mutation in human cardiomyocytes.

Molecular therapy. Nucleic acids·2025

Same author

Neuropathy-associated Tecpr2 mutation knock-in mice reveal endolysosomal loss of function phenotypes in neurons and microglia.

Cell death & disease·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Genome Editing in Mice Using TALE Nucleases.

Benedikt Wefers^1,2, Christina Brandl^3,4, Oskar Ortiz⁵

¹Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany. benedikt.wefers@helmholtz-muenchen.de.

Methods in Molecular Biology (Clifton, N.J.)

|October 8, 2015

Summary

This summary is machine-generated.

Gene editing in mouse zygotes using Transcription Activator-Like Effector Nucleases (TALENs) allows for rapid generation of targeted mouse mutants. This method bypasses the need for embryonic stem cells, streamlining genetic research.

Keywords:

Gene targeting Knock-in Knockout Mouse One-cell embryo TALEN

More Related Videos

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

Related Experiment Videos

Last Updated: Apr 1, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

Area of Science:

Biomedical Research
Molecular Biology
Genetics

Background:

Gene engineering is crucial for creating targeted mouse mutants in biomedical research.
Traditional methods often require embryonic stem cells, which can be time-consuming and complex.

Purpose of the Study:

To provide protocols for applying Transcription Activator-Like Effector Nucleases (TALENs) in mouse zygotes.
To demonstrate a streamlined method for generating targeted mouse mutants.

Main Methods:

Utilizing TALENs as sequence-specific nucleases to induce targeted double-strand breaks in mouse zygotes.
Employing embryo microinjection of TALEN mRNAs and targeting vectors.
Directly modifying the mouse genome in zygotes.

Main Results:

Successful generation of knockout and knock-in alleles in mouse zygotes.
Demonstration of fast and efficient genome modification without embryonic stem cells.
Establishment of protocols for TALENs application in mouse zygotes.

Conclusions:

TALENs offer a powerful and efficient tool for direct genome modification in mouse zygotes.
This technology significantly accelerates the generation of targeted mouse mutants for research.
The provided protocols facilitate the application of TALENs in mouse zygote gene engineering.