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Related Concept Videos

Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

6.6K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
6.6K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.0K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

4.3K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
4.3K
Mass Spectrometers01:16

Mass Spectrometers

11.0K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
11.0K
Mass Spectrometry: Alkene Fragmentation00:59

Mass Spectrometry: Alkene Fragmentation

3.9K
Alkenes lose one electron from the unsaturated π bond upon ionization and form stable molecular ions. Further fragmentation of alkenes occurs through three different reaction pathways. The most prominent fragmentation is the cleavage at the allylic position. The resultant allylic carbocation is resonance stabilized. In the mass spectra of terminal alkenes, this fragment appears at a mass-to-charge ratio of 41. In the internal alkenes, where there are two choices of allylic cleavage, the...
3.9K
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation01:01

Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation

2.8K
The fragmentation patterns observed for compounds such as carboxylic acids, esters, and amides in the mass spectra include ⍺-cleavage and McLafferty rearrangement. Fragmentation by ⍺-cleavage preferentially occurs at the carbon-carbon bond at the ⍺-position next to the carboxylic group to generate a neutral radical and a cation. Long chain compounds with hydrogen at their γ-carbon undergo McLafferty rearrangement to give a radical cation and a neutral alkene.
For example, the...
2.8K

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Related Experiment Video

Updated: Apr 1, 2026

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
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Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

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Electron-based fragmentation methods in mass spectrometry: An overview.

Yulin Qi1, Dietrich A Volmer1

  • 1Institute of Bioanalytical Chemistry, Saarland University, Campus B2.2, Saarbrücken, 66123, Germany.

Mass Spectrometry Reviews
|October 8, 2015
PubMed
Summary

Electron capture dissociation (ECD) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) offers advanced biomolecular structural analysis. This review highlights ECD fragmentation techniques and their optimization for FTICR mass analyzers.

Keywords:
ExDFTICR-MSfragmentationion-electron reactions

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Tandem mass spectrometry (MS/MS) is crucial for biomolecular structural characterization.
  • Electron capture dissociation (ECD) is an advanced MS/MS technique.
  • Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) offers high resolution and sensitivity.

Conclusions:

  • ECD is a powerful tool for biomolecular structural elucidation when used with FTICR-MS.
  • Further exploration of ECD techniques can advance mass spectrometry applications.
  • This review serves as an introduction to the potential of ECD in mass spectrometry research.