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Updated: Apr 1, 2026

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
Published on: June 19, 2017
Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors
Hicham Drissi1,2,3, Jason D Gibson4, Rosa M Guzzo4,5
1Department of Orthopaedic Surgery, UConn Health, 263 Farmington Avenue, Farmington, CT, 06030-4037, USA. drissi@uchc.edu.
Abstract:
The induction of human embryonic stem cells to a mesenchymal-like progenitor population constitutes a developmentally relevant approach for efficient directed differentiation of human embryonic stem (hES) cells to the chondrogenic lineage. The initial enrichment of a hemangioblast intermediate has been shown to yield a replenishable population of highly purified progenitor cells that exhibit the typical mesenchymal stem cell (MSC) surface markers as well as the capacity for multilineage differentiation to bone, fat, and cartilage. Herein, we provide detailed methodologies for the derivation and characterization of potent mesenchymal-like progenitors from hES cells and describe in vitro assays for bone morphogenetic protein (BMP)-2-mediated differentiation to the chondrogenic lineage.
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