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Related Experiment Videos

Growing and developing Dictyostelium cells express different ras genes.

S M Robbins1, J G Williams, K A Jermyn

  • 1Department of Microbiology, University of British Columbia, Vancouver, Canada.

Proceedings of the National Academy of Sciences of the United States of America
|February 1, 1989
PubMed
Summary

Dictyostelium discoideum has two ras genes. DdrasG is expressed during growth, while Ddras is expressed during later development, suggesting distinct roles for these ras proteins.

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Area of Science:

  • Cellular and Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The cellular slime mold Dictyostelium discoideum exhibits developmentally regulated gene expression.
  • Previously, a single ras gene (Ddras) was identified, primarily expressed during the pseudoplasmodial stage.

Purpose of the Study:

  • To identify and characterize additional ras genes in Dictyostelium discoideum.
  • To investigate the developmental expression patterns of these ras genes.

Main Methods:

  • Isolation of cDNA clones using molecular cloning techniques.
  • Analysis of gene expression patterns through developmental stages.

Main Results:

  • Discovery of a second ras gene, DdrasG, encoding a protein similar to Ddras.

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  • DdrasG expression is restricted to growth and early developmental phases, contrasting with Ddras expression.
  • Dictyostelium discoideum uniquely expresses different ras genes at distinct developmental stages.

    • Conclusions:

    • The two ras genes, DdrasG and Ddras, likely have divergent functions.
    • DdrasG may be involved in cell growth, while Ddras could play a role in multicellular development and signal transduction.