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Updated: Apr 1, 2026

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Published on: February 9, 2009
Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established
Anja Serve1, Michael Martin Pieler1, Dirk Benndorf2
1Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstrasse 1, 39106 Magdeburg, Germany.
Abstract:
A method for the purification of influenza virus particles using novel magnetic sulfated cellulose particles is presented and compared to an established centrifugation method for analytics. Therefore, purified influenza A virus particles from adherent and suspension MDCK host cell lines were characterized on the protein level with mass spectrometry to compare the viral and residual host cell proteins. Both methods allowed one to identify all 10 influenza A virus proteins, including low-abundance proteins like the matrix protein 2 and nonstructural protein 1, with a similar impurity level of host cell proteins. Compared to the centrifugation method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis.

