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A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity
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A novel bispecific antibody, S-Fab, induces potent cancer cell killing

Li Li1, Ping He, Changhua Zhou

  • 1*School of Pharmaceutical Sciences †Centre for Cellular & Structural biology, Sun Yat-Sen University, Guangzhou ‡Peking University Cancer Hospital, Beijing Institute for Cancer Research §General Staff Department of PLA, Health Division of Guard Bureau, Beijing, China.

Journal of Immunotherapy (Hagerstown, Md. : 1997)
|October 9, 2015
PubMed

Bispecific antibodies that engage immune cells to kill cancer cells have been actively studied in cancer immunotherapy. In this study, we present a novel bispecific format, S-Fab, fabricated by linking a single-domain anti-carcinoembryonic antigen VHH to a conventional anti-CD3 Fab. In contrast to most bispecific antibodies, the S-Fab bispecific antibody can be efficiently expressed and purified from bacteria. The purified S-Fab is stable in serum and is able to recruit T cells to drive potent cancer cell killing. In xenograft models, the S-Fab antibody suppresses tumor growth in the presence of human immune cells. Our study suggested that the bispecific S-Fab format can be applied to a wide range of immunotherapies.

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