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Updated: Apr 1, 2026

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Published on: February 9, 2009
Identification of molecular determinants for the nuclear import of pseudorabies virus UL31
Meili Li1, Si Jiang1, Chuncong Mo2
1Department of Pathogenic Biology and Immunology, School of Basic Science, Guangzhou Medical University, Guangzhou 511436, PR China; Guangzhou Hoffmann Institute of Immunology, School of Basic Science, Guangzhou Medical University, Guangzhou 511436, PR China.
Abstract:
Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infection. Pseudorabies virus (PRV) UL31 is a late protein homologous to HSV-1 UL31. Previous studies showed that PRV UL31 is predominantly localized to nucleus, however, the molecular determinants for its nuclear import were unclear to date. Here, by utilizing live cells fluorescent microscopy, UL31 fused with enhanced yellow fluorescent protein was transiently expressed in live cells and confirmed to exclusively target to the nucleus in the absence of other viral proteins. Furthermore, the nuclear import of UL31 was found to be dependent on the Ran-, importin α1-, α3-, α5-, α7-, β1-and transportin-1-mediated pathway. Therefore, these results would open up new avenues for depicting the biological functions of UL31 during PRV infection.
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