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An Interface for the Direct Coupling of Small Liquid Samples to AMS.

T J Ognibene1, A T Thomas1, P F Daley1

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Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
|October 13, 2015
PubMed
Summary

A new moving wire interface enables Accelerator Mass Spectrometry (AMS) analysis of liquid samples. This technology precisely measures small amounts of carbon-14 (14C) in biochemical research, expanding capabilities for analyzing minute samples.

Keywords:
14CCO2HPLC interfacesaccelerator mass spectrometry

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Nuclear Science

Background:

  • Accelerator Mass Spectrometry (AMS) is a powerful technique for isotope ratio measurements.
  • Analysis of nonvolatile liquid samples, especially in biomedical research, presents unique challenges for AMS.

Purpose of the Study:

  • To introduce and characterize a novel moving wire interface for AMS analysis of nonvolatile liquid samples.
  • To enable the analysis of discrete liquid drops and direct output from chromatographic separations.
  • To expand the capabilities of AMS for biomedical applications requiring the measurement of trace biochemicals.

Main Methods:

  • Development and implementation of a moving wire interface coupled to a 1-MV AMS system.
  • Analysis of discrete liquid samples and samples from high-performance liquid chromatography (HPLC).
  • Quantification of carbon (C) and radiocarbon (14C) content with focus on precision and dynamic range.

Main Results:

  • The system can measure discrete samples with as little as 50 zeptomoles (zmol) of 14C.
  • Achieved precision of 3-5% for 14C measurements in minutes.
  • Demonstrated a dynamic range of approximately three orders of magnitude.
  • Minimized sample-to-sample memory effects through optimized procedures.

Conclusions:

  • The moving wire interface is a viable technology for the AMS analysis of nonvolatile liquid samples.
  • This advancement significantly enhances the capacity for low-level biochemical analysis in extremely small samples.
  • The technology is expected to broaden the scope of biomedical AMS research, particularly for trace analyte detection.