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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
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Updated: Apr 1, 2026

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
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A candidate reference method using ICP-MS for sweat chloride quantification.

Jake T Collie, R John Massie, Oliver A H Jones

    Clinical Chemistry and Laboratory Medicine
    |October 13, 2015
    PubMed
    Summary
    This summary is machine-generated.

    A new Inductively Coupled Plasma Mass Spectrometry (ICP-MS) method accurately quantifies sweat chloride (Cl) for cystic fibrosis (CF) diagnosis. The off-line internal standard approach offers superior accuracy, making ICP-MS a strong candidate reference method.

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    Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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    Area of Science:

    • Analytical Chemistry
    • Clinical Diagnostics
    • Mass Spectrometry

    Background:

    • Cystic Fibrosis (CF) diagnosis relies on sweat chloride (Cl) quantification.
    • Existing methods require improvement for accuracy and standardization.
    • A candidate reference method is needed for the Joint Committee for Traceability in Laboratory Medicine (JCTLM).

    Purpose of the Study:

    • To develop and validate an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) method for sweat Cl quantification.
    • To present the ICP-MS method as a candidate reference method for CF diagnosis.
    • To compare on-line and off-line internal standard (IS) approaches within the ICP-MS method.

    Main Methods:

    • Developed two ICP-MS methods for sweat Cl analysis, one using on-line IS and another using off-line IS (Gallium).
    • Validated the methods for linearity, accuracy, and imprecision.
    • Participated in the Royal College of Pathologists of Australasia Quality Assurance Program (RCPAQAP) for sweat electrolyte testing.

    Main Results:

    • Achieved linearity up to 225 mmol/L with a limit of quantitation of 7.4 mmol/L.
    • The off-line IS method showed higher accuracy (CV 1.9%, bias 1.5 mmol/L) compared to the on-line IS method (CV 8.0%, bias 3.8 mmol/L).
    • Both methods demonstrated excellent reproducibility and linearity against RCPAQAP target values.

    Conclusions:

    • ICP-MS is a highly accurate and reproducible method for sweat Cl analysis with a low limit of quantitation.
    • The off-line IS ICP-MS method is recommended to minimize pre-analytical errors in CF diagnosis.
    • The developed ICP-MS method is a strong candidate reference method for CF monitoring and diagnosis.