Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.3K
Histone Modification02:32

Histone Modification

17.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
17.2K
Histone Modification02:32

Histone Modification

4.9K
4.9K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.7K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.7K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

15.2K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
15.2K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

10.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forces for Folding.

Acta naturae·2021
Same author

Thermodynamic basis of the α-helix and DNA duplex.

European biophysics journal : EBJ·2021
Same author

Redefinition of the cleavage sites of DNase I on the nucleosome core particle.

Journal of molecular biology·2004
Same author

Detection of micrometastases in lymph nodes from patients with breast cancer.

The British journal of surgery·2002
Same author

Characterization of an SRY-like gene, DSox14, from Drosophila.

Gene·2001
Same author

DNA looping in the RNA polymerase I enhancesome is the result of non-cooperative in-phase bending by two UBF molecules.

Nucleic acids research·2001
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Related Experiment Video

Updated: Apr 1, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
14:40

Expression Analysis of Mammalian Linker-histone Subtypes

Published on: March 19, 2012

14.3K

Linker histones: History and current perspectives.

C Crane-Robinson1

  • 1Biophysics Laboratories, School of Biology, University of Portsmouth, PO1 2DT, UK.

Biochimica Et Biophysica Acta
|October 14, 2015
PubMed
Summary
This summary is machine-generated.

The fifth histone (H1) helps condense chromatin, but its precise location and mechanism of action require further investigation. Understanding H1

Keywords:
ChromatinLinker histoneNucleosome

More Related Videos

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
08:12

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Published on: May 5, 2022

4.6K
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

11.6K

Related Experiment Videos

Last Updated: Apr 1, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
14:40

Expression Analysis of Mammalian Linker-histone Subtypes

Published on: March 19, 2012

14.3K
Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
08:12

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Published on: May 5, 2022

4.6K
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

11.6K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Chromatin Structure

Background:

  • The overall structure of the fifth histone (linker histone, H1) is known.
  • H1 plays a role in chromatin condensation.
  • The precise location and function of H1 on the nucleosome are not fully understood.

Purpose of the Study:

  • To clarify the exact location of H1 on the nucleosome.
  • To determine the mechanisms by which H1 achieves chromatin condensation.
  • To investigate the role of H1 in gene regulation and its dynamic deposition/removal.

Main Methods:

  • The abstract does not specify the methods used.
  • Further research is needed to determine the methods.

Main Results:

  • H1 is essential for chromatin fiber condensation.
  • H1 is not a global gene repressor; its displacement is required for transcription initiation.
  • There is only partial loss of H1 along the gene body during transcription.

Conclusions:

  • The precise mechanisms of H1-mediated chromatin condensation need further elucidation.
  • The dynamic processes of H1 deposition and removal are critical areas for future study.
  • Linker histones (H1) are abundant nuclear proteins with incompletely understood functions.