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Updated: Oct 25, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Forces for Folding
1Biophysics Laboratories, School of Biology, University of Portsmouth, PO1 2DT, UK.
Abstract:
Understanding the nature of the forces driving the folding of proteins, nucleic acids and the formation of their complexes absolutely requires thermodynamic data, in addition to structural information. In practical terms, this means the use of super-sensitive scanning and titration calorimeters for experimental determination of the heats (enthalpies) characterising these processes. Peter Privalov was both an experimental thermodynamicist and a calorimeter designer/manufacturer who followed and propagated this credo. The sum total of his many publications, every one of which addresses a fundamental question, is his lasting epitaph.
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