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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Leaky Scanning02:28

Leaky Scanning

5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Translation in Prokaryotes01:29

Translation in Prokaryotes

2.5K
Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...
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Translational Regulation01:29

Translational Regulation

819
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
819

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Related Experiment Video

Updated: Mar 31, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

29.1K

Studying the Translatome with Polysome Profiling.

Paola Zuccotti1, Angelika Modelska2

  • 1Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Via delle Regole 101, 38123, Mattarello, Trento, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2015
PubMed
Summary

Polysome profiling, a technique analyzing RNA and protein, reveals changes in mRNA translation and genome-wide translatome effects. This study details the sucrose gradient centrifugation method and subsequent isolation procedures.

Keywords:
PolysomeProfilingProtein isolationRNA isolationSucrose gradientTranscriptomeTranslational controlTranslatome

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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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Polysome Profiling in Leishmania, Human Cells and Mouse Testis

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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

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Related Experiment Videos

Last Updated: Mar 31, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

29.1K
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

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Area of Science:

  • Molecular Biology
  • Translational Control

Background:

  • Understanding mRNA translation is crucial for cellular function.
  • Genome-wide analysis of translation provides insights into gene expression regulation.

Purpose of the Study:

  • To describe the polysome profiling technique.
  • To detail RNA and protein isolation from sucrose fractions for translational analysis.

Main Methods:

  • Sucrose density gradient centrifugation for polysome fractionation.
  • Analysis of RNA and protein from isolated polysome fractions.

Main Results:

  • Successful fractionation of polysomes.
  • Isolation of RNA and protein from sucrose gradient fractions.

Conclusions:

  • Polysome profiling is a powerful technique for studying translational regulation.
  • The described methods enable comprehensive analysis of the translatome.