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Related Experiment Video

Updated: Mar 31, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

Identifying RBP Targets with RIP-seq.

Hans-Herman Wessels¹, Antje Hirsekorn¹, Uwe Ohler¹

¹Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|October 15, 2015

Summary

This summary is machine-generated.

RNA-binding proteins (RBPs) regulate gene expression by interacting with RNA molecules. Identifying these RNA-RBP interactions is crucial for understanding gene regulation.

Keywords:

Immunoprecipitation RNA-binding protein RNA-seq Ribonucleoprotein

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Related Experiment Videos

Last Updated: Mar 31, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

RNA-binding proteins (RBPs) are essential regulators of gene expression.
RBPs influence RNA processes including splicing, polyadenylation, editing, transport, stability, and translation.
The human genome encodes approximately 1500 RBPs, with recent studies identifying around 1100 proteins that interact with polyadenylated RNA.

Purpose of the Study:

To highlight the significance of RNA-RBP interactions in gene expression regulation.
To underscore the importance of identifying specific RNA targets for each RBP.

Main Methods:

This study is a review and synthesis of existing research on RNA-binding proteins and their interactions.
It analyzes data from recent studies that have identified proteins interacting with polyadenylated RNA.

Main Results:

A substantial number of proteins (~1100) have been found to directly interact with polyadenylated RNA.
The vast repertoire of RBPs (~1500 in the human genome) suggests complex regulatory networks.

Conclusions:

Identifying the specific RNA molecules bound by individual RBPs is critical for a comprehensive understanding of gene regulation.
Further research into RNA-RBP interactions will continue to yield key insights into cellular processes.