Has-miR-30a regulates autophagic activity in cervical cancer upon hydroxycamptothecin exposure
Yanxiang Cheng1, Gantao Chen2, Min Hu1
1Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
Abstract:
Cervical cancer is a leading cause of morbidity and mortality in women worldwide. Hydroxycamptothecin (HCPT) represents a new generation of antitumor agents targeting DNA topoisomerase I, which has been applied to treat various cancers with fewer side effects. Autophagy is emerging as an important biological mechanism in targeting human cancers, including cervical cancer. In this study, We have reported that HCPT could induce autophagy in Hela cells. Our investigation of the underlying mechanisms revealed that the decreased expression of miR-30a is involved in HCPT-induced autophagy. Futhermore, we showed that miR-30a could directly target a specific fragment in the 3' untranslated region of Beclin-1 as demonstrated by luciferase assay and overexpression of hsa-miR-30a by transfecting with miR-30a mimic could block HCPT-induced autophagic activity. It is the first time provide a deeper understanding of the mechanisms underlying cellular and molecular mechanisms by which HCPT affect cervical cancer.
More Related Videos
Related Concept Videos
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Autophagic Cell Death
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Drugs that Stabilize Microtubules
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
Inhibition of Cdk Activity
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...


