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Molecular identification and phylogenetic analysis of Metarhabditis blumi (Nematoda: Rhabditida)
Patrícia Vieira Bossi1, Erika Aparecida Consoli2, Juliana Magrinelli Osório Rosa2
1Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Abstract:
The species Metarhabditis blumi was diagnosed for the first time in Brazil using a molecular assay. Parasites isolated from two cattle herds from the state of Minas Gerais, southeastern Brazil, were classified as M. blumi according to their D2/D3 expansion fragments of the 28S rDNA sequence, which had a high degree of homology (100% similarity) with a M. blumi sequence deposited in GenBank (EU195965.1). The phylogenetic tree comparison also confirmed grouping of the populations evaluated in the current study as M. blumi.
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