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A General Method for Measuring Persister Levels in Escherichia coli Cultures
Niilo Kaldalu1, Arvi Jõers1, Henri Ingelman1
1Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.
Methods in Molecular Biology (Clifton, N.J.)
|October 16, 2015
Abstract:
Genetically homogeneous bacterial cultures contain persisters, cells that are not killed by bactericidal antibiotics. These cells are suggested to be involved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.
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