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Updated: Mar 31, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
Published on: May 2, 2019
Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA
Kristen L Kozielski1,2, Jordan J Green3,4,5,6,7
1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Abstract:
RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequence-specific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications.
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