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ROS Live Cell Imaging During Neuronal Development
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Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure-Function Dynamics
Omer Ziv1, Assaf Zaritsky2, Yakey Yaffe1
1Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel.
Plos Computational Biology
|October 17, 2015
Summary
Neural stem cell (NSC) dynamics within neural rosettes were quantified using live imaging. Early rosettes show enhanced radial organization and faster movements, while later rosettes exhibit decreased organization and slower dynamics, impacting brain development.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Neural stem cells (NSCs) are crucial for brain development.
- Understanding NSC cytoarchitectural dynamics in vivo is challenging.
- Neural rosettes derived from human pluripotent stem cells offer a model for studying NSC behavior.
Purpose of the Study:
- To develop a quantitative live imaging framework to characterize interkinetic nuclear migration (INM) dynamics in neural rosettes.
- To investigate the relationship between NSC cytoarchitecture, rosette development, and INM.
- To explore the impact of molecular perturbations on INM.
Main Methods:
- Quantitative live imaging of neural rosettes derived from human pluripotent stem cells.
- Characterization of apical-basal interkinetic nuclear migration (INM) dynamics.
- Analysis of radial organization, motion patterns, and temporal stability of INM.
- Molecular perturbation studies involving actin and non-muscle myosin-II (NMII) inhibition.
Main Results:
- Radial organization of NSCs within rosettes correlates with rosette size and mechanical constraints.
- Early-forming rosettes exhibit faster NSC motion and enhanced radial organization, mirroring early cortical development.
- Later-derived rosettes show slower motion, decreased radial organization, and temporal instability in INM, reflecting reduced NSC capacity and neurogenesis.
- Inhibition of actin or NMII significantly reduced INM measures.
Conclusions:
- The developed framework enables quantitative assessment of NSC cytoarchitectural dynamics in neural rosettes.
- NSC dynamics and radial organization change predictably during rosette development, correlating with proliferative and neurogenic phases.
- These findings have implications for understanding brain development, drug screening, and disease modeling using iPS cell-based platforms.
