Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells

Area of Science:

• Veterinary Oncology
• Cancer Cell Biology

Background:

• Celecoxib and citrate exhibit antitumor properties in various cancer cells.
• Antitumor efficacy of these agents in canine mammary tumors requires further investigation.

Purpose of the Study:

• To investigate the apoptotic and antiproliferative effects of citrate and celecoxib, individually and in combination, on the CF41—Mg canine mammary tumor cell line.

Main Methods:

• Cell viability was assessed using the MTT assay.
• Apoptosis induction was evaluated via Annexin—PI staining.
• Experiments were conducted on the CF41—Mg canine mammary tumor cell line.

Main Results:

• Both single and combination treatments significantly inhibited tumor growth in a dose-dependent manner.
• Citrate and celecoxib demonstrated significant apoptosis induction at their respective IC50 concentrations after 48 hours.
• Combined treatment showed enhanced toxicity and apoptosis induction at lower concentrations, with a decrease in necrotic cells.

Conclusions:

• Coadministration of citrate and celecoxib exhibits significant cytotoxic and apoptotic effects on CF41—Mg cells.
• This combination therapy presents a promising strategy for combating cancer cell proliferation and overcoming chemoresistance in canine mammary tumors.