Comparison of Sensitivities and Specificities of ELISA and Histopathology to Diagnose Feline Infectious Peritonitis

Feline infectious peritonitis (FIP) is one of the most prevalent viral infectious diseases in cats. It presents a number of challenges for veterinarians in terms of diagnosis. The objective of this study was to compare the sensitivity and specificity of ELISA with that of histopathology. Samples were obtained from 28 cats exhibiting signs consistent with feline infectious peritonitis (FIP) at the northwest animal clinics in Tehran, Iran, between January 2013 and 2015. Of the cats examined, five were deemed healthy, 14 exhibited indications of wet FIP, and nine displayed symptoms of dry FIP. Furthermore, the sensitivities and specificities of biochemical parameters were determined. The sensitivity and specificity of the ELISA test for diagnosing effusive FIP were found to be 100%, which was identical to the results obtained from histopathology. The AST (AUC=0.708) and total bilirubin (AUC=0.74) demonstrated moderate clinical accuracy in diagnosing FIP. The optical densities (ODs) in positive cats and the negative control group exhibited no statistically significant difference between the effusive and non-effusive forms of FIP. The Youden index was employed to determine the optimal cut-off point for the ratio of ODs in positive and negative cats, which was estimated to be 3.375. In conclusion, the ELISA demonstrated high predictive values for the diagnosis of effusive FIP and has the potential for use in the serological diagnosis of feline coronavirus infection.