Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision 3D profilometry of consumer-grade computer enclosures using high dynamic range fringe projection.

Applied optics·2026
Same author

Encrypted fringe projection profilometry.

Optics letters·2026
Same author

FDCGAN: frequency domain constrained generative adversarial network for overexposed fringe image restoration.

Optics express·2025
Same author

4D line-scan hyperspectral imaging.

Optics express·2021
Same author

Fringe projection profilometry by conducting deep learning from its digital twin.

Optics express·2020
Same author

Motion-induced error reduction for binary defocusing profilometry via additional temporal sampling.

Optics express·2019
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Mar 31, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.5K

Flexible calibration method for microscopic structured light system using telecentric lens.

Beiwen Li, Song Zhang

    Optics Express
    |October 20, 2015
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method for calibrating microscopic structured light systems. The novel approach achieves high accuracy for 3D measurements using a telecentric lens camera.

    More Related Videos

    Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
    08:32

    Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

    Published on: January 26, 2024

    3.7K
    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
    09:43

    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

    Published on: March 20, 2017

    10.4K

    Related Experiment Videos

    Last Updated: Mar 31, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.5K
    Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
    08:32

    Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

    Published on: January 26, 2024

    3.7K
    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
    09:43

    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

    Published on: March 20, 2017

    10.4K

    Area of Science:

    • Metrology
    • Optical Engineering
    • Computer Vision

    Background:

    • Microscopic 3D measurement systems require precise calibration for accurate results.
    • Structured light systems are widely used for 3D reconstruction but can be challenging to calibrate accurately, especially at microscopic scales.
    • Telecentric lenses offer advantages in microscopic imaging by minimizing perspective distortion, but their integration into calibration procedures needs careful consideration.

    Purpose of the Study:

    • To present a novel and accurate calibration method for a microscopic structured light system.
    • To integrate a camera with a telecentric lens into the structured light calibration framework.
    • To achieve high-precision 3D coordinate measurements within a defined microscopic volume.

    Main Methods:

    • The proposed method calibrates the projector using standard pin-hole camera calibration procedures.
    • Iterative Levenberg-Marquardt optimization is employed to estimate 3D coordinates of feature points.
    • A mathematical model for a telecentric lens is utilized for camera calibration.

    Main Results:

    • The developed calibration framework demonstrates very high accuracy.
    • Achieved accuracy is approximately 10 micrometers.
    • The system successfully calibrated for a measurement volume of approximately 10 mm (H) x 8 mm (W) x 5 mm (D).

    Conclusions:

    • The novel calibration method provides a robust solution for microscopic structured light systems.
    • The integration of telecentric lens cameras enhances measurement accuracy and precision.
    • This research contributes to advancements in high-accuracy 3D metrology at microscopic scales.