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    Area of Science:

    • Optical microscopy
    • Holography
    • Biomedical imaging

    Background:

    • Traditional holographic microscopes can be complex and costly.
    • Previous work utilized a dual-pinhole aperture for holographic microscopy.

    Purpose of the Study:

    • To develop a low-cost, ultra-compact holographic microscope.
    • To enable simultaneous imaging of multiple areas with high resolution and accuracy.
    • To explore the use of a multi-pinhole aperture for enhanced holographic microscopy.

    Main Methods:

    • Employed a multi-pinhole aperture to generate reference and multiple object waves.
    • Interference of waves on a digital sensor to form multiplexed off-axis holograms.
    • Eliminated the need for lenses, splitters, or combiners in the system design.

    Main Results:

    • Successfully retrieved quantitative phase and amplitude images from multiple imaging areas simultaneously.
    • Achieved a lateral resolution of approximately 2.2µm.
    • Demonstrated optical path accuracy in the tens of nanometers.

    Conclusions:

    • The multi-pinhole aperture is optimal for this compact holographic microscope system.
    • The system offers a cost-effective and efficient solution for advanced imaging.
    • Findings provide insights for traditional multiplexed off-axis holography.