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Updated: Mar 31, 2026

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Comparative Transcriptomics in Yeasts.

Dawn A Thompson¹

¹Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA. dawnt@broadinstitute.org.

Methods in Molecular Biology (Clifton, N.J.)

|October 21, 2015

Summary

We present a new experimental framework for comparative transcriptomics, enabling deeper insights into the evolution of gene expression across diverse species and complex phylogenetic relationships.

Area of Science:

Evolutionary biology
Genomics
Transcriptomics

Background:

Comparative functional genomics illuminates gene expression evolution and phenotypic diversity.
Challenges exist in comparing gene expression across numerous species within a phylogeny.

Purpose of the Study:

To introduce an experimental framework for comparative transcriptomics.
To address challenges in analyzing gene expression evolution in complex phylogenies.

Main Methods:

Development of a novel experimental framework.
Application of comparative transcriptomics.
Analysis across a complex phylogenetic tree.

Main Results:

The framework facilitates robust comparative transcriptomic analysis.

Keywords:

Comparative expression profiling Evolution Gene regulation

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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It enables the study of gene expression evolution in complex evolutionary histories.

Conclusions:

The proposed framework is essential for advancing our understanding of gene expression evolution.
It provides a scalable approach for multi-species transcriptomic comparisons.