Related Experiment Video
Updated: Mar 31, 2026
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
A Unique Plasmodium falciparum K13 Gene Mutation in Northwest Ethiopia
Abebe Genetu Bayih1, Gebeyaw Getnet1, Abebe Alemu1
1Department of Pathology and Laboratory Medicine, University of Calgary, Alberta, Canada; Department of Medical Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia; School of Medicine, College of Health Sciences and Medicine, Wolaita Sodo University, Wolaita, Ethiopia; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Alberta, Canada; Department of Medicine, University of Calgary, Alberta, Canada.
Abstract:
Artemisinin combination therapy (ACT) is the first line to treat uncomplicated Plasmodium falciparum malaria worldwide. Artemisinin treatment failures are on the rise in southeast Asia. Delayed parasite clearance after ACT is associated with mutations of the P. falciparum kelch 13 gene. Patients (N = 148) in five districts of northwest Ethiopia were enrolled in a 28-day ACT trial. We identified a unique kelch 13 mutation (R622I) in 3/125 (2.4%) samples. The three isolates with R622I were from Negade-Bahir and Aykel districts close to the Ethiopia-Sudan border. One of three patients with the mutant strain was parasitemic at day 3; however, all patients cleared parasites by day 28. Correlation between kelch 13 mutations and parasite clearance was not possible due to the low frequency of mutations in this study.
More Related Videos
09:13Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases
Published on: November 22, 2024
09:25CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
Published on: September 18, 2018