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Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Genomic DNA in Prokaryotes

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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Returning to more finished genomes.

Jonas Korlach¹

¹Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, United States.

|October 21, 2015

Summary

This summary is machine-generated.

Longer reads from single molecule, real-time (SMRT) sequencing offer a more complete view of genomes. This technology overcomes the fragmentation common with short-read sequencing, improving genomic data analysis.

Keywords:

Consensus accuracy DNA sequencing De novo assembly GC bias Reference genome Sequence read length

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Genomic data is crucial in biological sciences, transforming research methodologies.
Second-generation sequencing technologies predominantly yield short-read data, leading to fragmented and incomplete genomic information.
Fragmented genomic data presents challenges in comprehensive analysis and understanding.

Purpose of the Study:

To highlight the advantages of long-read sequencing technologies.
To emphasize the potential of single molecule, real-time (SMRT) sequencing for resolving complex genomes.
To advocate for the adoption of SMRT sequencing for more contiguous genomic data.

Main Methods:

Review of current sequencing technologies and their limitations.
Discussion of the capabilities of single molecule, real-time (SMRT) sequencing.
Analysis of how long, unbiased reads improve genome assembly and characterization.

Main Results:

SMRT sequencing generates long, high-quality reads.
Long reads enable the assembly of more complete and contiguous genomes.
This approach mitigates the fragmentation issues associated with short-read data.

Conclusions:

Single molecule, real-time (SMRT) sequencing provides a superior method for obtaining comprehensive genomic data.
The shift towards long-read sequencing facilitates a return to holistic genome analysis.
This technology is essential for advancing biological research through complete genomic insights.