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An update of the macaque testis proteome
Tao Zhou1, Yueshuai Guo1, Zuomin Zhou1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, PR China.
Data in Brief
|October 21, 2015
Summary
This study refined the macaque testis proteome, identifying thousands of proteins and providing evidence for predicted genes. This updated proteogenomic dataset aids in annotating the rhesus macaque genome and understanding spermatogenesis.
Area of Science:
- Genomics
- Proteomics
- Reproductive Biology
Background:
- The rhesus macaque genome is a draft with errors and incomplete Y chromosome annotation.
- Previous macaque testis proteome data requires refinement and integration with updated genomic information.
Purpose of the Study:
- To reanalyze and enhance the macaque testis proteome using the latest genomic data.
- To identify refined protein sequences, potential Y chromosome proteins, and validate predicted transcripts.
- To provide a high-quality proteogenomic dataset for rhesus macaque genome annotation and spermatogenesis research.
Main Methods:
- Reanalysis of a previously published macaque testis proteome dataset.
- Searching against updated Ensembl protein sequences for macaque.
- Identification and grouping of peptides to protein level.
Main Results:
- Identification of 74,433 peptides, corresponding to 9,247 protein groups.
- Provided peptide-level evidence for predicted genes and transcripts.
- Generated an updated macaque testis proteome dataset.
Conclusions:
- The updated macaque testis proteome offers improved proteogenomic annotation capabilities.
- This dataset is valuable for studying the mechanisms of rhesus macaque spermatogenesis.
- The findings contribute to a more accurate understanding of the macaque genome, particularly the Y chromosome.
