Related Experiment Video
Updated: Mar 31, 2026
Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
Published on: April 25, 2025
Comparative analysis of CD4 and CD8 lymphocytes - evidences for different distribution in primary and secondary liver
Simona E Giuşcă, Piotr M Wierzbicki, Cornelia Amălinei
1Department of Morphofunctional Sciences - Histology, "Grigore T. Popa" University of Medicine and Pharmacy, Iaşi, Romania. irinadragacaruntu@gmail.com.
Introduction:
The impact of tumor cells on tumor-infiltrating lymphocytes (TILs) in cancer development is not yet clarified. Our study analyzed the distribution and prognostic value of CD4+ and CD8+ T lymphocytes in hepatocellular carcinoma (HCC) and liver metastases (LM).
Material And Methods:
Archival tissue specimens of 35 HCC and 39 LM patients were immunohistochemically processed. The number of intratumoral (IT) and peritumoral (PT) CD4+ and CD8+ T cells was quantitatively analyzed.
Results:
We noted large variances of T lymphocyte subpopulations. Similar number of CD4+ and CD8+ lym-phocytes was present in HCC, whereas in LM the number of CD8+ cells was approximately two times higher than CD4+ lymphocytes. A significant prevalence of T cells in PT over IT areas was observed. The prognostic value was demonstrated only for PT CD8+ lymphocytes in LM, their reduced number being associated with shorter survival.
Conclusions:
The differences between proportions of T lymphocytes within tumor and its environment might be explained by proapoptotic effect of cancer cells on TILs.
