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Related Experiment Video

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Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
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The Cytocam video microscope. A new method for visualising the microcirculation using Incident Dark Field technology.

Sam Hutchings1,2, Sarah Watts1, Emrys Kirkman1

  • 1Biophysics division, Defence Science and Technology Laboratory, Porton Down, Salisbury, UK.

Clinical Hemorheology and Microcirculation
|October 21, 2015
PubMed
Summary

The new Braedius Cytocam device offers comparable microcirculatory assessment to older technology. This incident dark field (IDF) microscope provides better vessel contrast for improved analysis in critical care settings.

Keywords:
Microcirculationhaemorrhagic shockincident dark field imagingsidestream dark field imaging

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Area of Science:

  • Medical Devices
  • Microcirculation Research
  • Critical Care Technology

Background:

  • Assessing microcirculation is crucial for understanding tissue perfusion in critical conditions.
  • Side stream dark field (SDF) imaging has been used for microcirculatory assessment.
  • Advancements in imaging technology aim to improve diagnostic capabilities.

Purpose of the Study:

  • To introduce and evaluate the Braedius Cytocam, a novel incident dark field (IDF) video microscope.
  • To compare the performance of the Braedius Cytocam with a precursor device using SDF imaging.
  • To assess the accuracy of microcirculatory measurements in both baseline and shock states.

Main Methods:

  • The Braedius Cytocam (IDF) and a precursor SDF device were used for simultaneous measurements.
  • Sublingual microcirculation of pigs was assessed under baseline and hemorrhagic shock conditions.
  • Image analysis focused on total vessel density (TVD), microvascular flow index (MFI), and image quality, including vessel contrast.

Main Results:

  • Both devices demonstrated comparable total vessel density and microvascular flow index at baseline and during shock.
  • No significant bias was found between the IDF and SDF devices using Bland and Altman analysis.
  • The Braedius Cytocam (IDF) showed significantly superior vessel contrast compared to the SDF device in both capillaries and venules.

Conclusions:

  • The Braedius Cytocam provides comparable vessel detection to precursor SDF devices.
  • The new IDF technology offers enhanced vessel contrast, potentially improving microcirculatory assessment.
  • The device is suitable for evaluating microcirculation in critical care scenarios, including shock states.