Natural Carriers for siRNA Delivery.

Area of Science:

• Biotechnology and Nanomedicine
• Drug Delivery Systems
• Gene Therapy

Background:

• Effective delivery of small interfering RNA (siRNA) is critical for gene silencing therapies.
• Natural carriers offer a promising alternative to synthetic or viral vectors for siRNA delivery.
• The evolution of carriers from historical viral vectors to modern nanostructures is highlighted.

Purpose of the Study:

• To review and analyze carriers of natural origin for siRNA delivery.
• To discuss the advantages and disadvantages of various natural carrier systems.
• To explore modifications that enhance the efficiency of siRNA delivery.

Main Methods:

• Comprehensive literature review of natural carrier systems for siRNA.
• Analysis of polysaccharides (chitosan, alginates), proteins, and cell-derived entities (exosomes, mini cells).
• Evaluation of carrier modifications and their impact on delivery efficiency.

Main Results:

• Polysaccharides like chitosan and alginates have demonstrated success in siRNA delivery.
• Natural peptides and their mimics represent versatile carrier options.
• Cell-derived vesicles, including exosomes and mini cells, are emerging as advanced natural carriers.

Conclusions:

• Natural carriers, particularly polysaccharides, peptides, and cell-derived vesicles, are vital for effective siRNA delivery.
• Modifications to these carriers can significantly improve therapeutic outcomes.
• Further research into natural carriers holds substantial promise for advancing gene therapy applications.