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Complexation of Lysozyme with Sodium Poly(styrenesulfonate) via the Two-State and Non-Two-State Unfoldings of
Fu-Gen Wu1,2, Yao-Wen Jiang2, Hai-Yuan Sun1
1Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University , Beijing 100084, People's Republic of China.
Abstract:
To provide an in-depth understanding of the complexation mechanism of protein and polyelectrolyte, a heating-cooling-reheating protocol was employed to study the unfolding and refolding behaviors of a model protein, lysozyme, in the presence of a negatively charged polyelectrolyte, sodium poly(styrenesulfonate) (PSS). It was found that, with elevated PSS concentration, a new state (state I) was first formed via a "two-state" conversion process and this state could further convert to a completely unfolded state (state II) via a "non-two-state" conversion. This non-two-state conversion process occurs without the coexistence of states I and II but involves the formation of various intermediate unfolded protein structures. Different from the pure lysozyme that exhibited refolding upon cooling from its heat-denatured state, lysozyme in state I could undergo unfolding upon heating but no refolding upon cooling, while lysozyme in state II did not undergo unfolding or refolding upon thermal treatments. In addition, the effects of ionic strength and molecular weight of polyelectrolyte on the unfolding and refolding behaviors of lysozyme were also investigated. The present work provides a better understanding of the principles governing protein-polyelectrolyte interactions and may have implications for the fabrication of biocolloids and biofilms.
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