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Bone core biopsies. Plastic or paraffin?

L Vincic¹, S Weston, R H Riddell

¹Department of Pathology, McMaster University Medical Center, Hamilton, Ontario, Canada.

The American Journal of Surgical Pathology

|April 1, 1989

Summary

This summary is machine-generated.

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Semi-thin paraffin sections offer excellent bone core biopsy morphology, rivaling plastic embedding. This method is more cost-effective and allows for crucial peroxidase studies, making it ideal for routine diagnostics.

Area of Science:

Histopathology
Biopsy Analysis
Bone Marrow Examination

Background:

Plastic embedding of bone core biopsies is favored for superior morphology via semi-thin sections (1-2 μm).
However, plastic embedding presents challenges including increased cost, technical difficulty, and limitations with special stains and immunoperoxidase studies.

Purpose of the Study:

To evaluate if semi-thin paraffin sections (1-2 μm) can achieve comparable morphological results to plastic embedding.
To assess the feasibility and advantages of using paraffin sections for bone core biopsy analysis.

Main Methods:

45 bone core biopsy specimens were divided, with one half processed using plastic embedding and the other using paraffin embedding after decalcification.
Both halves were sectioned at 1-2 μm for morphological comparison.

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Main Results:

High-quality semi-thin paraffin sections (1-2 μm) are routinely achievable, comparable to plastic sections.
Paraffin sections demonstrated advantages including cost-effectiveness, reduced technologist time, and successful performance of peroxidase studies.
Peroxidase studies on paraffin sections were vital for diagnosing myeloma and identifying metastatic carcinoma.

Conclusions:

Semi-thin paraffin sections (1-2 μm) provide excellent morphological detail for bone core biopsies.
Paraffin embedding is recommended for routine bone core biopsy examination due to its cost-effectiveness, technical simplicity, and compatibility with essential diagnostic techniques like peroxidase staining.