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Updated: Mar 31, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and
Ines Zidi-Jrah1, Amani Hajlaoui1, Soumaya Mougou-Zerelli1
1Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Objective:
To study the possible relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss (RPL).
Design:
Descriptive study.
Setting:
University-affiliated tertiary teaching.
Patient(S):
A total of 22 couples with history of RPL and 20 fertile men.
Intervention(S):
Semen samples from case and control men were examined for differences in semen parameters, DNA fragmentation, chromatin condensation, and sperm aneuploidy.
Main Outcome Measure(S):
Sperm DNA and chromatin integrity and sperm aneuploidy.
Result(S):
Sperm progressive motility (30.2% vs. 51.5%) was significantly lower and abnormal morphology (74.8% vs. 54.2%) was significantly higher in the RPL group versus the control group, respectively. The percentage of fragmented DNA was significantly increased in the RPL group (17.1% vs. 10.2%) as well as the rate of spermatozoa with nuclear chromatin decondensation (23.6% vs. 11.8%). There was a significantly higher sperm aneuploidy rate among the RPL group as well.
Conclusion(S):
The increase in abnormal sperm parameters, sperm DNA fragmentation, nuclear chromatin decondensation, and sperm aneuploidy suggest possible causes of unexplained RPL.
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