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[Simultaneous Determination of Five Active Components in Puerariae Lobatae Radix from Different Habitats by UHPLC]
Objective:
To develop a UHPLC method for determination of 3'-hydroxy puerarin,puerarin, 3'-methoxy puerarin, daid- zin, and daidzein in Puerariae Lobatae Radix from different habitats.
Methods:
The analysis was carried out on an Agela Venusil MP C18 (100 mm x 2. 1 mm, 3 µm) column. The mobile phase was composed of 0. 1% formic acid and methanol with gradient elution. The de- tection wavelength was set at 250 nm.
Results:
The standard curves of five components showed a good linearity in 12. 41 ~ 248. 24 ng(r =0. 9999), 58. 82 ~ 1 176. 47 ng(r =0. 9997), 12. 65 ~252. 94 ng(r=0. 9999), 12.14 ~ 242.82 ng(r=0. 9998), and 1. 82 ~ 36.30 ng(r =0. 9997), respectively. The average recoveries were 99. 03% ~ 100. 32%, and RSD values were 0. 26% ~ 1. 37%.
Conclusion:
This method is simple, quick,reproducible and can be used to control the quality of Puerariae Lobatae Radix.
