Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Active versus Passive Immunity01:31

Active versus Passive Immunity

12.0K
Immunity, along with the ability to limit pathogen growth to prevent significant body tissue damage, can be gained either by (1) actively developing an immune response within the individual after exposure to a pathogen or after getting vaccinated or (2) passively transferring immune components from an immune individual to one who is nonimmune. Both these forms of immunity can be found naturally and in medical practices.
Active Immunity
Active immunity refers to the resistance one develops...
12.0K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

18.4K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
18.4K
Vaccinations01:51

Vaccinations

53.9K
Overview
53.9K
Humoral Immune Responses01:36

Humoral Immune Responses

86.3K
Overview
86.3K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

17.4K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
17.4K
Antigen Processing Pathways01:31

Antigen Processing Pathways

3.0K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of West Nile disease convalescents identifies human monoclonal antibodies protective against West Nile and related orthoflaviviruses.

Immunity·2026
Same author

Neutrophil regulation of immunotherapy for cancer is controlled by type II interferon.

Immunity·2026
Same author

Human antibodies against West Nile and related orthoflaviviruses.

bioRxiv : the preprint server for biology·2026
Same author

Selective depletion of virus-specific CD8 T cells from the liver after PD-1 therapy with Fc-intact antibody during chronic infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Stabilized mosaic hemagglutinin immunogens as novel universal influenza virus vaccines.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Mechanistic Basis for the Selective Recognition of the Fcγ Receptor IIa by Monoclonal Antibody IV.3.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 31, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
04:47

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

8.3K

Fcγ receptor pathways during active and passive immunization.

Stylianos Bournazos1, Jeffrey V Ravetch1

  • 1The Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York, NY, USA.

Immunological Reviews
|October 27, 2015
PubMed
Summary

Immunoglobulin G (IgG) antibodies use their Fc domain to interact with Fc gamma receptors (FcγRs) on immune cells. This interaction is crucial for mediating immune responses during both active and passive immunization.

Keywords:
Fc receptorsantibodiesimmunotherapiesinflammationvaccination

More Related Videos

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

9.4K
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

22.7K

Related Experiment Videos

Last Updated: Mar 31, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
04:47

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

8.3K
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

9.4K
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

22.7K

Area of Science:

  • Immunology
  • Molecular Biology
  • Structural Biology

Background:

  • Immunoglobulin G (IgG) antibodies are critical for adaptive immunity, mediating effector functions through their bifunctional structure.
  • The variable Fab domain provides antigen specificity, while the constant Fc domain interacts with Fc gamma receptors (FcγRs) on leukocytes.
  • FcγR engagement initiates diverse downstream signaling pathways that modulate immune cell activity and antibody responses.

Purpose of the Study:

  • To explore the structural heterogeneity of the IgG Fc domain and its impact on FcγR interactions.
  • To elucidate how Fc domain structure, including subclass variations and N-linked glycosylation, influences FcγR binding affinity and downstream signaling.
  • To highlight the central role of IgG-FcγR interactions in modulating adaptive immunity during active and passive immunization.

Main Methods:

  • Analysis of IgG subclass structural differences.
  • Investigation of Fc-associated N-linked glycan composition.
  • Assessment of IgG Fc domain conformational flexibility.
  • Studies on the differential binding of IgG Fc domains to various FcγR types (Type I and Type II).

Main Results:

  • The IgG Fc domain exhibits significant structural heterogeneity due to subclass variations and N-linked glycosylation.
  • These structural determinants modulate Fc domain conformation, affecting interactions with different FcγRs.
  • Differential FcγR engagement leads to diverse immunomodulatory outcomes, including cytotoxicity, phagocytosis, and T-cell modulation.

Conclusions:

  • IgG Fc domain structure and glycosylation are key regulators of FcγR-mediated effector functions.
  • Understanding IgG-FcγR interactions is vital for comprehending adaptive immunity and optimizing immunotherapy strategies.
  • These interactions play a pivotal role in both active and passive immunization processes.