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Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications
C Krafft1, I W Schie1, T Meyer2
1Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany. juergen.popp@ipht-jena.de.
Chemical Society Reviews
|October 27, 2015
Summary
Raman scattering microscopy offers advanced biomedical imaging. This technique provides detailed insights into cells, tissues, and whole animals for diverse applications.
Area of Science:
- Biomedical optics
- Vibrational spectroscopy
- Microscopy
Background:
- Raman scattering is a label-free vibrational spectroscopic technique.
- It provides molecular contrast for imaging.
- Recent advancements have enhanced its applicability in biomedicine.
Purpose of the Study:
- To introduce Raman-based techniques for biomedical applications.
- To provide an overview of spontaneous and coherent Raman scattering microscopy instrumentation.
- To summarize imaging strategies and present biomedical applications.
Main Methods:
- Overview of spontaneous Raman scattering (SpRS) and coherent Raman scattering (CRS) microscopy instrumentation.
- Discussion of recent developments in Raman microscopy hardware.
- Summary of imaging strategies: sequential registration, line imaging, and wide-field imaging.
Main Results:
- Raman-based techniques show significant potential in biomedicine.
- Recent instrumentation developments enable advanced imaging capabilities.
- Diverse applications demonstrated across various biological samples.
Conclusions:
- Raman scattering microscopy is a powerful tool for biomedical research.
- Its versatility spans from single cells to whole animals.
- Further development promises expanded diagnostic and research capabilities.
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