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Related Experiment Video

Updated: Mar 31, 2026

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

9.3K

Transgenic mouse offspring generated by ROSI.

Pedro Moreira1, Serafín Pérez-Cerezales, Ricardo Laguna

  • 1Departamento de Reproducción Animal, INIA, Madrid 28040, Spain.

The Journal of Reproduction and Development
|October 27, 2015
PubMed
Summary

Researchers developed a novel method for creating transgenic animals using round spermatid oocyte injection (ROSI). This technique efficiently produces transgenic mice, demonstrating that immature sperm nuclei can be used for gene transfer.

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Area of Science:

  • Reproductive biology
  • Genetics
  • Animal biotechnology

Background:

  • Transgenic animal production is crucial for research and applications.
  • Existing methods include microinjection, gene transfer, and nuclear transfer.
  • A need exists for efficient and alternative transgene delivery systems.

Purpose of the Study:

  • To introduce a new method for producing transgenic animals.
  • To report the successful production of transgenic mice using round spermatid oocyte injection (ROSI).
  • To investigate the efficiency and applicability of ROSI for gene transfer.

Main Methods:

  • Co-injection of DNA and round spermatid nuclei into non-fertilized mouse oocytes (ROSI).
  • Utilized a transgene construct with the human CMV promoter and enhanced GFP gene.

Related Experiment Videos

Last Updated: Mar 31, 2026

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

9.3K
  • Analyzed transgene integration, expression, and offspring viability.

    • Main Results:

    • Achieved a 12% efficiency in producing transgenic offspring via ROSI.
    • ROSI efficiency was comparable to pronuclear injection and ICSI.
    • Demonstrated Egfp mRNA and EGFP fluorescence in multiple organs of ROSI-generated transgenic mice.
    • Confirmed that immature sperm nuclei with decondensed chromatin can mediate transgene incorporation.

    Conclusions:

    • ROSI is an effective method for generating transgenic animals.
    • The study expands the understanding of gene transfer mechanisms using immature gametes.
    • ROSI offers a viable alternative for transgene delivery into oocytes.