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SIS/PDGF-B expression in benign and malignant human breast lesions
1Department of Medical Oncology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Oncogene
|March 1, 1989
Summary
High expression of the SIS/PDGF-B gene was found in human breast carcinomas. This gene expression, particularly in larger tumors, may contribute to the stromal reaction observed in breast lesions.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The role of growth factors in breast cancer development is crucial.
- Specific gene expression patterns can indicate tumor progression and stromal interactions.
Purpose of the Study:
- To investigate the expression of the SIS/PDGF-B gene in primary human breast carcinomas.
- To determine the cellular localization of SIS/PDGF-B expression.
- To explore the potential link between SIS/PDGF-B expression and stromal reactions in breast lesions.
Main Methods:
- Northern blotting was used to analyze RNA expression.
- Poly(A)-selected RNAs from four primary human breast carcinomas were examined.
- In situ hybridization with photobiotinylated probes was performed for cellular localization.
Main Results:
- High expression of a 3.7 kb SIS/PDGF-B transcript was detected in three out of four breast carcinoma samples.
- The identified tumors were large (T3, >5 cm) infiltrating ductal carcinomas with significant desmoplasia.
- SIS/PDGF-B expression was localized to epithelial cells in both malignant and benign breast lesions.
Conclusions:
- SIS/PDGF-B is highly expressed in a subset of human breast carcinomas, particularly larger ones.
- The localization of SIS/PDGF-B expression within epithelial cells suggests a role in influencing the tumor microenvironment.
- Epithelial SIS/PDGF-B expression may contribute to the desmoplastic stromal reaction observed in breast lesions.