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Updated: Mar 31, 2026

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
Published on: May 5, 2022
Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament
1Department of Pathology, Emory University, 615 Michael Street, Whitehead Research Building, Room 105N, Atlanta, GA, 30322, USA. sono@emory.edu.
Abstract:
Dynamics of actin filaments are regulated by a number of actin-binding proteins. To understand the function of an actin-binding protein, it is necessary to characterize effects of the protein on actin filament dynamics in vitro. This chapter describes basic microscopic methods to visualize fluorescently labeled actin filaments using commonly available fluorescence microscope settings. Direct microscopic observation of actin filaments provides strong evidence for severing or bundling of actin filaments.
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