Related Experiment Video
Updated: Aug 9, 2026
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
Published on: August 24, 2010
Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila
Michael Gotesman1, Selwyn A Williams2
1Department of Biology, Technion - Israel Institute of Technology, Technion, Haifa, 3200003, Israel. mgotesman@tx.technion.ac.il.
Methods in Molecular Biology (Clifton, N.J.)
|October 27, 2015
Abstract:
This chapter describes protocols for using a handheld gene gun to deliver transformation vectors for overexpression of genes or gene replacement in the macronucleus of Tetrahymena thermophila. The protocols provide helpful information for preparing Tetrahymena for biolistic bombardment, preparation of vector-coated microcarriers, and basic gene gun operating procedures.
Related Concept Videos
00:53Transgenic Organisms
Overview
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...