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Updated: Mar 31, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Molecular typing of XDR Acinetobacter baumannii strains in an Italian ICU
Andrea Piana1,2, Alessandra Palmieri3,2, Silvia Deidda3
1Dip. Scienze biomediche, igiene e medicina preventiva, Università di Sassari, Italy. piana@uniss.it.
Objective:
To investigate the antimicrobial susceptibility and clonal relationship of Acinetobacter baumannii strains isolated in an Italian ICU.
Design:
Epidemiological, observational, retrospective, longitudinal study.
Setting And Participants:
The ICU of the University Hospital of Sassari, Italy.
Main Outcome Measures:
Pulsed Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST) were used to evaluate the genomic features of the isolated strains.
Results:
Drug susceptibility testing for all isolated strains showed the same resistance pattern, characterized by resistance to the most important antibiotics, with the only exception of colistin. PFGE showed a very poor between-strain variability; three distinct clusters, 11, 4, and 1 isolates in size, were identified (Dice's coefficient: 92.11%). MLST showed that all isolated strains belonged to sequence type 2 (ST2). All isolates collected from the environment and the human samples were positive for the following genes: blaOXA-23, blaOXA-51-like, blaVIM-like, blaIMP-like, andISAba1; however, blaOXA-24-like, blaOXA-58-like, and blaNDM-like were not detected.
Conclusions:
The survey identified XDR strains belonging to the same cell clone, confirming the wide circulation and environmental persistence of this microorganism.
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