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Updated: Mar 31, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

A High-Throughput Radiometric Kinase Assay.

Krisna C Duong-Ly¹, Jeffrey R Peterson²

¹Cancer Biology Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2015

Summary

This summary is machine-generated.

This study presents a robust dot blot kinase assay for screening small-molecule compounds against protein kinases. This high-throughput method aids in identifying kinase inhibitors and targets, including the insulin receptor kinase.

Keywords:

Dot blot kinase assay High-throughput screen Insulin receptor Kinase Kinase assay Kinase inhibitor

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Last Updated: Mar 31, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Assaying the Kinase Activity of LRRK2 in vitro

Assaying the Kinase Activity of LRRK2 in vitro

Published on: January 18, 2012

Area of Science:

Biochemistry
Pharmacology
Drug Discovery

Background:

Aberrant kinase signaling is linked to various diseases, making kinases attractive drug targets.
Despite their importance, validated inhibitors exist for only a small fraction of human protein kinases.
Screening compound libraries is crucial for identifying kinase inhibitors and targets.

Purpose of the Study:

To present a protocol for a dot blot kinase assay.
To demonstrate its utility for measuring insulin receptor kinase activity.
To highlight its adaptability for other protein kinases.

Main Methods:

Utilized a dot blot kinase assay format.
Employed high-throughput screening of small-molecule compounds.
Focused on measuring insulin receptor kinase activity.

Main Results:

The dot blot kinase assay is a robust method for screening compounds against kinases.
The protocol is effective for measuring insulin receptor kinase activity.
The assay can be readily adapted for various protein kinases.

Conclusions:

The dot blot kinase assay is a valuable tool in kinase inhibitor development.
This high-throughput assay facilitates the identification of novel kinase inhibitors and targets.
The presented protocol offers a versatile approach for kinase research.