Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to correspondence: "Reappraising the neurovascular link between arterial stiffness and neuropathy: proposal for a neurovascular uncoupling index".

Journal of hypertension·2026
Same author

The Association Between Adherence to the Dutch Healthy Diet Index and Glaucoma Prevalence-The Maastricht Study.

Nutrients·2026
Same author

Association of tear fluid glutathione synthetase and glutathione levels with amyloid positivity.

Scientific reports·2026
Same author

Uncovering the Genetic Architecture of Optic Nerve Integrity Estimates through Genome-wide Association Study Meta-analyses.

medRxiv : the preprint server for health sciences·2026
Same author

Circulating eNAMPT in Glaucoma: A Semi-Quantitative Plasma Analysis Before and After Nicotinamide Supplementation.

Translational vision science & technology·2026
Same author

Comparing the surgical outcomes of femto laser-assisted cataract surgery (FLACS) versus conventional phacoemulsification (CP) in nanophthalmic eyes.

Indian journal of ophthalmology·2025

Related Experiment Video

Updated: Mar 31, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

4.0K

Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes.

Yuan Tian1, Aize Kijlstra1, Johan Renes2

  • 1University Eye Clinic Maastricht, Postbus 5800, 6202 AZ Maastricht, Netherlands.

Journal of Ophthalmology
|October 28, 2015
PubMed
Summary
This summary is machine-generated.

Lutein supplementation may lower circulating factor D (FD) levels by inhibiting its production in fat cells. This finding could explain previous observations in age-related macular degeneration (AMD) patients.

More Related Videos

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC
06:28

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

20.5K
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
05:34

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

Published on: January 24, 2025

3.3K

Related Experiment Videos

Last Updated: Mar 31, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

4.0K
Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC
06:28

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

20.5K
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
05:34

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

Published on: January 24, 2025

3.3K

Area of Science:

  • Ophthalmology
  • Immunology
  • Nutritional Science

Background:

  • Age-related macular degeneration (AMD) pathogenesis involves the complement system.
  • Factor D (FD), a key enzyme in the complement alternative pathway, is decreased by lutein supplementation.
  • Adipose tissue synthesizes FD and stores lutein.

Purpose of the Study:

  • To investigate if lutein affects factor D (FD) synthesis in adipocytes.
  • To explore the mechanism behind reduced circulating FD levels after lutein supplementation.

Main Methods:

  • Mature human adipocytes were cultured and treated with lutein (50 μg/mL).
  • Factor D (FD) mRNA and protein expression were measured after 24 and 48 hours of incubation.

Main Results:

  • Lutein significantly inhibited FD mRNA expression in adipocytes.
  • Lutein reduced FD protein release into the cell culture supernatant.

Conclusions:

  • Lutein may block adipocyte production of factor D (FD).
  • This inhibition could explain the observed decrease in circulating FD levels in individuals supplemented with lutein, potentially impacting AMD progression.