Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.3K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial Adsorption Enhancement between C-S-H and Microcapsule Walls (Ethyl Cellulose/SiO<sub>2</sub>): Mechanisms from Molecular Dynamics and Experiments.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

A Radial Modulus-Gradient Fiber for Chronic Recording and Decoding in Deep Brain.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Enhancing biomedical optical volumetric imaging via self-supervised orthogonal learning.

Science advances·2026
Same author

Multilateration-based photoacoustic tomography for reconstruction-free 3D particle localization.

Optics express·2026
Same author

Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.

Nanophotonics (Berlin, Germany)·2026
Same author

Thermo-Responsive Self-Recoverable Porous Sensors with Writable Electrodes: Advancing Wearable Motion Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Related Experiment Video

Updated: Mar 31, 2026

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
13:21

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Published on: November 20, 2010

28.5K

Isolating single cells in a neurosphere assay using inertial microfluidics.

S Shiva P Nathamgari1, Biqin Dong, Fan Zhou

  • 1Department of Theoretical and Applied Mechanics, Northwestern University, Evanston, IL 60208, USA. espinosa@northwestern.edu.

Lab on a Chip
|October 30, 2015
PubMed
Summary

This study introduces a novel microfluidic method to efficiently separate single stem cells from clusters, crucial for accurate analysis. The technique preserves cell viability and multipotency, enabling advanced stem cell research.

More Related Videos

Preparation of Neuronal Co-cultures with Single Cell Precision
09:06

Preparation of Neuronal Co-cultures with Single Cell Precision

Published on: May 20, 2014

14.4K
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
09:08

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Published on: September 13, 2018

10.4K

Related Experiment Videos

Last Updated: Mar 31, 2026

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
13:21

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Published on: November 20, 2010

28.5K
Preparation of Neuronal Co-cultures with Single Cell Precision
09:06

Preparation of Neuronal Co-cultures with Single Cell Precision

Published on: May 20, 2014

14.4K
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
09:08

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Published on: September 13, 2018

10.4K

Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Microfluidics

Background:

  • Sphere-forming assays are vital for stem cell research but yield heterogeneous cell populations.
  • Dissociated neurospheres contain unwanted cell clusters, hindering clonal analysis and differentiation studies.
  • Existing microfluidic methods often operate at high flow rates, potentially damaging cells.

Purpose of the Study:

  • To develop a microfluidic technique for separating single cells from clusters in dissociated neurospheres.
  • To optimize sorting at low flow rates, minimizing shear stress and preserving cell viability.
  • To demonstrate the applicability of the method for downstream stem cell analysis and differentiation.

Main Methods:

  • Utilized inertial microfluidics with spiral channels operating at low flow rates.
  • Leveraged Dean's force to focus single cells and clusters based on size.
  • Chemically dissociated neurospheres were processed through the microfluidic device.

Main Results:

  • Achieved efficient separation of single cells from clusters with >90% viability.
  • Demonstrated preservation of neural stem cell multipotency through differentiation into neurons and astrocytes.
  • The low flow rate regime minimized shear stress on the cells.

Conclusions:

  • The developed microfluidic method offers a high-yield, low-stress approach for stem cell preparation.
  • This technique is adaptable for various sphere-forming assays and integration with other lab-on-a-chip systems.
  • Enables more accurate clonal analysis and differentiation studies of stem cells.