Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial Phase Coherence in Femtosecond Coherent Raman Scattering.

Physical review letters·2026
Same author

Multispectral coded light for high-speed temperature imaging of aluminum combustion.

Optics express·2026
Same author

Liquid volume fraction and droplet sizing in atomizing sprays using polarization ratio with dual structured laser illumination planar imaging: erratum.

Optics letters·2026
Same author

Engineered calcium-regulated affinity protein for efficient internalization and lysosomal toxin delivery.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Liquid volume fraction and droplet sizing in atomizing sprays using polarization ratio with dual structured laser illumination planar imaging.

Optics letters·2025
Same author

Configurable thermoacoustic streaming by laser-induced temperature gradients.

Physical review applied·2025

Related Experiment Video

Updated: Mar 31, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.5K

Single-shot photofragment imaging by structured illumination.

Kajsa Larsson, Malin Jonsson, Jesper Borggren

    Optics Letters
    |October 30, 2015
    PubMed
    Summary

    A new laser method uses structured illumination to suppress background noise in pump-probe measurements. This technique improves signal clarity for imaging, achieving a 20x increase in signal-to-interference ratio for single-shot flame diagnostics.

    More Related Videos

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.2K
    Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
    07:05

    Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

    Published on: June 20, 2025

    1.6K

    Related Experiment Videos

    Last Updated: Mar 31, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.5K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.2K
    Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
    07:05

    Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

    Published on: June 20, 2025

    1.6K

    Area of Science:

    • Laser-based diagnostics
    • Spectroscopy
    • Combustion chemistry

    Background:

    • Pump-probe measurements are crucial for studying transient species in flames.
    • Background interferences, such as naturally occurring radicals, can obscure signals from laser-induced photofragments.
    • Distinguishing between induced and natural signals is challenging in complex environments like flames.

    Purpose of the Study:

    • To present and demonstrate a novel laser method for suppressing background interferences in pump-probe measurements.
    • To enable, for the first time, single-shot imaging of HO(2) radicals in a flame.
    • To enhance the signal-to-interference ratio in challenging diagnostic scenarios.

    Main Methods:

    • Implementation of structured illumination by spatially modulating the pump beam's intensity profile.
    • Application of a spatial lock-in algorithm to extract encoded signal components.
    • Demonstration in a laminar methane/air flame for imaging OH photofragments.

    Main Results:

    • The structured illumination method effectively distinguishes pump-induced photofragment signals from background signals.
    • A significant increase in the signal-to-interference ratio (approximately 20-fold) was achieved for single-shot data.
    • Successful demonstration of the method's capability in a combustion environment.

    Conclusions:

    • The presented laser method offers a robust solution for background suppression in pump-probe spectroscopy.
    • This technique significantly improves the quality of diagnostic data, particularly for single-shot measurements.
    • The method holds promise for advancing the in-situ characterization of reactive species in flames and other complex systems.