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DNA polymorphisms in chickpea accessions as revealed by PCR-based markers
P Yadav1, K K Koul2, N Shrivastava3
1Jiwaji University School of Studies in Biotechnology Gwalior India.
Cellular and Molecular Biology (Noisy-Le-Grand, France)
|October 31, 2015
Summary
Genetic markers revealed diversity in chickpea (Cicer arietinum) germplasm, with ISSR markers showing higher correlation to pedigree data. Specific accessions are recommended for future breeding programs to enhance genetic variation.
Area of Science:
- Agricultural Science
- Genetics
- Plant Breeding
Background:
- Chickpea (Cicer arietinum) is a vital food legume, but its cultivated germplasm lacks sufficient genetic variation.
- Exploiting related annual and wild species is crucial for genetic improvement.
Purpose of the Study:
- To assess genetic polymorphism across diverse chickpea genotypes using molecular markers.
- To identify genetically diverse accessions for breeding programs.
Main Methods:
- Employed 42 Random Amplified Polymorphic DNA (RAPD) and 41 Inter Simple Sequence Repeat (ISSR) markers.
- Analyzed 20 chickpea genotypes from 10 global geographical areas.
- Utilized cluster analysis to determine genetic similarity and variability.
Main Results:
- RAPD markers detected 51% polymorphism; ISSR markers detected 54% polymorphism.
- ISSR markers showed a higher correlation with pedigree data compared to RAPD markers.
- Accessions from similar geographical origins exhibited greater genetic similarity.
Conclusions:
- Identified specific chickpea accessions (ICC6263, ICC6306, ICC17160) as valuable parents for breeding.
- Highlighted the need for further breeding programs to introduce additional genetic variation into chickpea stocks.
